Chub-s7
WebJun 1, 2024 · Methods: Differentiated human adipocytes (Chub-S7, n=6) were treated with a broccoli extract (BE; hybrid Brassica oleracea var. italic; 10ng/ml) alone or in combination with tunicamycin (Tun; 750ng/ml), an inducer of ER stress. ER stress genes (ATF4, ATF6 and CHOP) were measured at three time points (24hr, 48hr, 72hr, n=3) using qPCR. WebTo determine whether Chub-S7 cells were able to differenti-ate, the expression of adipocyte markers was assessed, by real-time RT-PCR, at different time points during cellular
Chub-s7
Did you know?
WebCellosaurus Chub-S7 (CVCL_0239) Doubling time: 45.6 +- 0.8 hours (PubMed= 12934077 ). Transfected with: UniProtKB; P03129; Human papillomavirus type 16 protein E7. … WebOct 11, 2016 · Methods and Results: Microarray analysis of human adipocytes (CHUB-S7 cell line) cultured and differentiated in customised media with varying concentrations of …
WebJul 1, 2013 · Influence of SQV on mitochondrial function in Chub-S7 cells. Oxygen consumption in Chub-S7 incubated with SQV. The routine rate of respiration was determined in intact cells by high resolution respirometry, using the energy substrates present in the medium. The non-differentiated and differentiated control Chub-S7 cells … WebMar 1, 2006 · In Chub-S7 cells, PPARγ expression, at day 17, was slightly enhanced by both T0901317 (2.8-fold) and BRL49653 (1.9-fold; Fig. 2), when compared to the basal condition.The combination of both agonists did not indicate an additive or synergistic effect on PPARγ expression (). aP2, like adiponectin, was stimulated by T0901317, but to a …
WebApr 4, 2024 · Our results are in line with those by Gathercole et al. , who reported increased insulin-stimulated glucose uptake in a human immortalized subcutaneous adipocyte line (Chub-S7) after acute exposure to dexamethasone, as well as to hydrocortisone (up to 48 hours, in a dose- and time-dependent manner for the latter), thus proposing that the ... WebApr 14, 2014 · After 17 days of differentiation, fully differentiated Chub-S7 cells were treated with the estrogen receptor alpha agonist 4,4′,4″- (4-Propyl- [1H]-pyrazole-1,3,5-triyl) trisphenol (PPT) (1426-Tocris, Bristol, U.K.) in DMEM media (D6046-Sigma-Aldrich). A vehicle DMSO control was included.
WebMay 15, 2024 · Chub-S7 adipocytes have been used for studying cellular metabolism (85), adipogenic differentiation, and miRs regulation (86). Other human preadipocyte cell lines were established through the...
WebOct 20, 2024 · Chub-s7 cells were cultured until 24h post-confluence and differentiation was induced by adding 1mM dexamethasone, 0.5mM IBMX, 10mg/ml of insulin and 10mM of rosiglitazone to the medium for 14-21 days, until the appearance of adipocyte morphology. For quantitative real-time PCR experiments, differentiated chub-s7 adipocytes were … flying instructor stormwindWebThe Chub-S7 cell line was derived from human subcutaneous primary preadipocytes which were transfected with human papillomavirus E7 oncoprotein and the human telomerase … flying instructor ratingWeb> chub s7 cells. micrococcal nuclease mnase About; News; Press Release; Team; Advisors; Partners; Contact; Bioz Stars; Bioz vStars; 86 : Buy from Supplier : Structured … green machine muffinsWebOverexpression or siRNA-mediated knockdown of endogenous C/EBPalpha significantly increased or decreased, respectively, adiponectin mRNA levels in differentiated human Chub-S7 adipocytes, while neither C/EBPbeta nor C/EBPdelta significantly affected adiponectin expression in mature adipocytes. green machine motorcycle gangWebBattery, heavy-duty 600 cold-cranking amps, maintenance-free with rundown protection and retained accessory power. Alternator, 145 amps (Standard on 3/4 ton models. Not … flying instructor salaryWebAug 22, 2003 · The immortalized Chub-S7 cells displayed a comparable proliferation rate and a similar doubling time to human primary stromal cells, 13 suggesting a limited deregulation of cell cycle checkpoints ... flying in tbc classicWebAug 22, 2003 · This cell line considered as immortal was named Chub-S7. Chub-S7 cells expressed HPV-E7 gene as detected by reverse transcription-polymeral chain reaction (RT–PCR) (data not shown). green machine motorized bicycle