Webshock protein, HSP150, of Saccharomyces cerevisiae, and the characterization of the gene coding for it. HSP150 is constitu-tively expressed, extensively 0-glycosylated, and secreted ef-ficiently to the growth medium. When cells grown at 25?C were shifted to 37?C, a 7-fold increase in the level of HSP150 was observed within 1 hr. WebWe have cloned and characterized the HSP150 gene of Saccharomyces cerevisiae, which encodes a glycoprotein (hsp150) that is secreted into the growth medium. Unexpectedly, the HSP150 gene was found to be regulated by heat shock and nitrogen starvation.
Enhancing Saccharomyces cerevisiae reactive oxygen species …
WebApr 9, 2004 · In the yeast Saccharomyces cerevisiae, environmental stress conditions that damage the cell wall lead to activation of the so-called “compensatory mechanism,” aimed at preserving cell integrity through a remodeling of this extracellular matrix.Here we used DNA microarrays to investigate the molecular basis of this response to two agents that induce … WebThe hsp150 delta-carrier is an N-terminal signal peptide-containing fragment of a yeast secretory glycoprotein. Hsp150 delta-NGFRe, harvested from the culture medium, … frostcrush valari
The contribution of the O‐glycosylated protein Pir2p/Hsp150 to …
WebUsing periplasmic disulfide-bonded β-lactamase of Escherichia coli as a reporter, we describe here the yeast-derived polypeptide Hsp150Δ, which promotes proper folding and ER exit of several foreign proteins in Saccharomyces cerevisiae and Pichia pastoris . Authentic Hsp150 (Fig. 1 A) mostly is secreted to the culture medium . The signal ... WebMay 1, 1992 · We report the finding of a secretory heat shock protein, HSP150, of Saccharomyces cerevisiae, and the characterization of the gene coding for it. HSP150 is constitutively expressed, extensively O-glycosylated, and secreted efficiently to … WebWe have cloned and characterized the HSP150 gene of Saccharomyces cerevisiae, which encodes a glycoprotein (hsp150) that is secreted into the growth medium. Unexpectedly, … ghs regulatory programs